In recent year, the analytical standards available for the standardization of fixed oils is insufficient as well as the standard finger print pattern is not available. Most of the studies of Ocimum spp. have been carried out with aqueous and non-aqueous extracts of leaves or volatile oil distilled from leaves. However, in the recent past, a considerable number of studies have been reported on fixed oil extracted from Ocimum spp. seeds. Thus the aim of this study was to extract and evaluate fixed oils from the seed of Ocimum sanctum and Ocimum basillicum which can be useful for designing new oil base formulations in future. The coarse powder of both Ocimum spp. seeds was extracted in petroleum ether (60-800) using continuous hot extraction method. The extract was taken out and petroleum ether was evaporated to obtain fixed oils. The resultant fixed oils were evaluated for analytical parameters such as physical constants and chemical constants. Thin Layer Chromatography (TLC) profile was also carried out to establish the chromatographic profile for the same. The study shows that, the parameters evaluated were within the permissible limits. This information can be useful for designing new formulations or for modification of the conventional formulations by using fixed oil as a base.
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